Effects of Dietary Supplementation of Saccharomyces cerevisiae on Testosterone, Serum Biochemistry and Performance of ISA Brown Cockerels (Gallus gallus domesticus)

This study investigated the effects of dietary Saccharomyces cerevisiae (SC) supplementation on testosterone, serum biochemistry, haematology, growth performance, and organ development in ISA Brown cockerels. Fifteen cockerels (six weeks old, 200–300 g) were randomly assigned to three groups designated A, B and C (n=5). Group A served as the control while groups B and C received 5 g/kg and 10 g/kg of SC respectively. Birds were fed standard grower mash for six weeks under uniform conditions. Testosterone was analyzed using a commercial (Enzyme-Linked Immunosorbent Assay (ELISA) kit, while serum biochemistry was assessed with an automated analyzer. Feed and water intake were monitored, and body weight was recorded weekly. Testosterone level was significantly higher (p<0.05) in group B. Supplemented groups showed improved urea levels and electrolyte profiles; however, group C had elevated creatinine and AST levels. Platelet counts increased significantly with dosage, while other haematological parameters remained unaffected. Feed intake and weight gain were significantly higher in group C from week 2 to 4 and from week 3 onward, respectively. Testicular weights increased in both supplemented groups, with the highest values in group C. In conclusion, SC supplementation at 5 g/kg improved testosterone level and physiological status, while 10 g/kg enhanced growth and testicular development but may induce metabolic strain. These findings highlight the importance of dose optimization to balance performance benefits with animal health.