Two Color TEM Labeling using Silver-Gold Intensification of DAB and CuDAB2 v1
This method is use for two color labeling of cell organelles for transmission electro microscopy. The color TEM labeling used Silver-Gold Intensification on polymerized DAB from photooxidation of miniSOG-mitomatrix and CuDAB2 labeling on DNA from oxidation of FeTAML-azide. The labeling were confirmed by EELS using a Gatan Imaging Filter on a ThermoFisher Talos 200C G2 transmission electron microscope at 200kV.
Authors
- Mark H. Ellisman (ORCID: https://orcid.org/0000-0001-8893-8455)
- Stephen Adams (ORCID: https://orcid.org/0000-0002-4725-2091)
- Mason Mackey
Institutions
- NCR (United States) (US)
Publication Details
- Published
- 2024-09-18
- DOI
- https://doi.org/10.17504/protocols.io.261ge5beog47/v1
- Primary Topic
- Cell Adhesion Molecules Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00