Mechanical and Antimicrobial Evaluation of Chitosan-Coated Elastomeric Orthodontic Modules

<b>Background/Objectives</b>: Orthodontic appliances disrupt oral biofilm homeostasis, leading to an increase in plaque and disease risk. Elastomeric modules (EMs) promote bacterial growth due to their material composition. Surface coatings have been developed to reduce bacterial colonization. We evaluated the mechanical, antimicrobial, and cell viability properties of a chitosan coating for EMs. <b>Methods</b>: EMs were coated with chitosan (CS) and chitosan-glutaraldehyde (CS-GTA) to assess antimicrobial and cell viability. Uncoated EMs were used as a control. These surface-coated modules were characterized and analyzed with Fourier transform infrared (FTIR) and Raman spectroscopy, and tensile testing. Antibacterial activity was assessed by colony-forming units (CFU) counts after incubation. Cell viability was tested with gingival fibroblasts using the MTT assay. ANOVA, Tukey, Kolmogorov-Smirnov, and Kruskal-Wallis tests were used for statistical analysis. <b>Results</b>: Raman spectra of the chitosan coatings showed characteristic molecular vibration bands. ANOVA revealed a significant difference in mechanical properties between the materials and between the control and the CS-GTA groups, confirmed by the Tukey post hoc test. No significant difference was observed between the groups in the Yield Stress test. All the coated groups showed reduced CFU counts in the antibacterial assay. The average cell viability of the coated groups was 85% and 89%. <b>Conclusions</b>: We synthesized CS and GTA-cross-linked chitosan coatings. The coatings did not affect the mechanical properties of the elastomeric modules. The chitosan and glutaraldehyde-cross-linked CS coatings inhibited bacterial growth. No significant differences were observed in antibacterial activity between the CS and the GTA-crosslinked chitosan coatings.