Nano-org, a functional resource for single-molecule localisation microscopy data
The nanoscale organisation of proteins plays a key role in diverse cellular processes, including signalling, adhesion, and structural integrity. Single-molecule localisation microscopy (SMLM) is a super-resolution imaging technique that captures the spatial distributions of proteins in cells with nanometre precision, enabling detailed studies of protein clustering and architecture. However, comparing such data across experiments remains challenging due to a lack of curated, functional resources. Here we present a publicly accessible, curated, and functional resource, termed "nano-org", containing SMLM data representing the nanoscale distributions of proteins in cells. Nano-org is searchable by comparing the statistical similarity of the datasets it contains. This unique functionality allows the resource to be used to understand the relationships of nanoscale architectures between proteins, cell types or conditions, supporting the development of the field of spatial nano-omics.
Authors
- Patrick Rubin‐Delanchy (ORCID: https://orcid.org/0000-0003-0577-0795)
- Khodor S. Hazime (ORCID: https://orcid.org/0000-0001-5125-2011)
- David J. Williamson (ORCID: https://orcid.org/0000-0002-6222-6297)
- Dylan M. Owen (ORCID: https://orcid.org/0000-0002-5284-2782)
- Ezra Bruggeman (ORCID: https://orcid.org/0000-0001-6100-1443)
- Daniel M. Davis (ORCID: https://orcid.org/0000-0002-9182-291X)
- Jeremy A. Pike (ORCID: https://orcid.org/0000-0003-4163-0335)
- Ruby Peters (ORCID: https://orcid.org/0000-0003-2946-7504)
- Steven F. Lee (ORCID: https://orcid.org/0000-0003-4492-5139)
- Daniel J. Nieves (ORCID: https://orcid.org/0000-0002-0873-4418)
- A. Garcia
- Mohammed Baragilly (ORCID: https://orcid.org/0000-0003-4775-4742)
- Jonathan D. Worboys (ORCID: https://orcid.org/0000-0002-8242-4901)
- Ricardo Henriques (ORCID: https://orcid.org/0000-0002-2043-5234)
- Mahmoud A. Ahmed (ORCID: https://orcid.org/0000-0003-3520-9157)
- S. Shirgill
- Kylie Savoye (ORCID: https://orcid.org/0009-0008-0111-8541)
- H. L. Abbott
Institutions
- Birmingham City University (GB)
- King's College London (GB)
- University of Exeter (GB)
- University of Cambridge (GB)
- Manchester Academic Health Science Centre (GB)
- University of Manchester (GB)
- MRC Laboratory for Molecular Cell Biology (GB)
- Centre for Inflammation Research (GB)
- University College London (GB)
- Imperial College London (GB)
- University of Birmingham (GB)
- Universidade Nova de Lisboa (PT)
- Helwan University (EG)
- University of Sheffield (GB)
- University of Edinburgh (GB)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-09-30
- DOI
- https://doi.org/10.1038/s41467-025-63674-x
- Citations
- 2
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 3.27
Funders
- European Commission
- University of Birmingham
- Directorate for Biological Sciences
- HORIZON EUROPE Framework Programme
- Medical Research Council
- Biotechnology and Biological Sciences Research Council