Generation of Polyclonal Antibodies Against Sabin Poliovirus D- and H-Antigens and Their Application in ELISA
This antigen-specific antibody approach provides a valuable tool for routine quality control in sIPV manufacturing, enabling accurate quantification of immunogenic components and detection of potentially immunogenic degradation products during vaccine storage and distribution. Antibodies to the D-antigen allow assessment of immunogenic, neutralizing epitopes, while antibodies to the H-antigen provide a tool for detecting non-neutralizing components. This antigen-specific antibody approach offers a valuable tool for studying the antigenic structure of sIPV and for improving the accuracy of ELISA-based antigen quantification.
Authors
- Olga Shmeleva
- Аnna A. Shishova (ORCID: https://orcid.org/0000-0002-5907-0615)
- Yury Y. Ivin (ORCID: https://orcid.org/0000-0003-0995-7944)
- Nikita Khabibullin (ORCID: https://orcid.org/0000-0002-1833-5840)
- И. С. Левин (ORCID: https://orcid.org/0000-0002-0800-575X)
- Aydar A. Ishmukhametov (ORCID: https://orcid.org/0000-0001-6130-4145)
- Irina O Tcelykh
- Vladislav Vasilenko (ORCID: https://orcid.org/0000-0003-0638-3383)
- S.Yu. Ivanov (ORCID: https://orcid.org/0000-0001-5458-0192)
- Anastasia N. Piniaeva (ORCID: https://orcid.org/0000-0001-5381-2393)
- Maya Yu. Ermakova (ORCID: https://orcid.org/0000-0002-8229-7818)
- Yulia V. Rogova (ORCID: https://orcid.org/0000-0002-4655-5330)
- Anastasia A. Kovpak (ORCID: https://orcid.org/0000-0003-3200-763X)
- Alla Kondrashova (ORCID: https://orcid.org/0000-0003-3993-6909)
- Anna N. Zyrina (ORCID: https://orcid.org/0000-0002-3675-8361)
- Alexandra A. Siniugina
- Nadezhda Borisenko
Institutions
- Russian Academy of Sciences (RU)
- Sechenov University (RU)
- Chumakov Institute of Poliomyelitis and Viral Encephalitides (RU)
Publication Details
- Journal
- Vaccines
- Published
- 2025-09-30
- DOI
- https://doi.org/10.3390/vaccines13101022
- Citations
- 2
- Primary Topic
- Viral Infections and Immunology Research
- Type
- article
- Field-Weighted Citation Impact
- 2.78