The Role of the Glyceraldehyde-3-phosphate Dehydrogenase and β-Amyloid Complex in the Activation of the Cellular Chaperone System
Objective: The aim of this study was to evaluate the cytotoxic effect of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-amyloid (Aβ1-42) co-aggregates on human mesenchymal cells reprogrammed into a neuronal phenotype. Methods: Co-aggregates were formed by incubating recombinant GAPDH and synthetic Aβ1-42 in the presence of tissue transglutaminase, which provides covalent cross-linking of proteins. Results: The data obtained demonstrate that GAPDH–Aβ1-42 co-aggregates have pronounced neurotoxicity, but are able to activate protective and compensatory mechanisms, including the chaperone apparatus. Conclusions: The results emphasize the need to develop therapeutic strategies aimed at blocking the GAPDH–Aβ1-42 interaction and supporting cellular protective pathways to prevent neurodegeneration
Authors
- Irina V. Guzhova (ORCID: https://orcid.org/0000-0002-8775-7713)
- Vladimir F. Lazarev (ORCID: https://orcid.org/0000-0002-7117-6789)
- Elizaveta A. Dutysheva (ORCID: https://orcid.org/0000-0003-0354-5199)
- Boris A. Margulis (ORCID: https://orcid.org/0000-0002-2608-0147)
- A. A. Sakharov
- A. V. Yurakova
Institutions
- Institute of Cytology (RU)
Publication Details
- Journal
- Cell and Tissue Biology
- Published
- 2025-09-30
- DOI
- https://doi.org/10.1134/s1990519x25060057
- Primary Topic
- Heat shock proteins research
- Type
- article
- Field-Weighted Citation Impact
- 0.00