Glutamin Enema Might Have Helped Reversing İntestinal Mucosal İnjury Among Diarrheic Calves: Trophic Aminoacid For ‘Microbiota-Gut-Brain Axis’

Introduction: Glutamine (Gln) has been denoted as an effectual nutritional supplementation for improving the gastrointestinal barrier function of animals. Aim: In the present study evaluating the efficacy of Gln on intestinal morphology, as detected by Diamine oxidase (dAo), a valuable marker for detecting gastrointestinal toxicity and intestinal mucosal injury, among diarrheic calves. Materials and Methods: In a total of 20 calves with diarrhea, with unknown etiology, were enrolled at this study. Following physical examination performed by board certified veterinary surgeons (who were not involved other than technical intervention), rapid diagnostic test kits were applied without any responsible etiological agent detected, a total of 0.9 ml blood was withdrawn from each calf. Sandwich ELISA by use of commercially available bovine dAo test kits were deemed available. Glutamine (Gut-cumin I Liquid Sol.) was given via rectal route as 15 mg/kg for 7 consequtive days. Results: Mean± SE dAo (ng/mL) analysis were deemed available at days 0 (start of trial) and primary end point (day 7), presented 9.12±2.46 vs. 28.19±3.18 (p