The Effect of Topiramate on Adiponectin Secretion in 3T3-L1 Adipocytes: An In Vitro Approach to Anti-Obesity Mechanisms

This study aimed to investigate the effects of topiramate, a pharmacological agent with emerging metabolic benefits on adipocyte differentiation and adipokine secretion, with a particular focus on adiponectin levels in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were induced to differentiate and subsequently treated with varying concentrations of topiramate (1–100 µM). Lipid accumulation was evaluated using Oil Red O staining, while adiponectin levels were measured via ELISA at 6, 12, and 24 hours. Cell viability was assessed using the MTT assay. Topiramate promoted significant lipid accumulation without cytotoxicity at concentrations up to 100 µM, with the 50 µM dose showing the highest lipid deposition. A significant increase in adiponectin secretion was observed in differentiated cells at 12 and 24 hours post-treatment (p