Antibacterial Effect of Plant-Based Bioactive Molecule Mixture Against Pseudomonas aeruginosa

The aim of this study was to determine the antibiotic resistance profile of Pseudomonas aeruginosa strains collected from the Microbiology Laboratory of Çukurova University Hospital and evaluate the antibacterial effect of a plant-based bioactive molecule (BAM) mixture against these strains. The VITEK-2 system and antibiogram cards were utilized for organism identification and antibiotic susceptibility determination. P. aeruginosa (N = 37) demonstrated high resistance rates to common antibiotics, including Ampicillin-Sulbactam (100%), Tetracycline (100%), Trimethoprim-Sulfamethoxazole (100%), Tigecycline (97.3%), and Imipenem (64.86%). Resistance was also observed for Levofloxacin, Piperacillin, and Netilmicin (59.46%), Meropenem and Amikacin (56.76%), Ciprofloxacin (40.54%), Cefoperazone-Sulbactam (32.43%), Ceftazidime (25%), Cefepime (24.32%), and Piperacillin-Tazobactam (14.29%). None of the strains were resistant to Colistin. BAM showed significant antibacterial activity, with the highest effect observed in strain P4 (99%). Activity was also notable in strains P2 (98%), P12 (97%), P11 (94%), P37 (81%), P14 (78%), and P1 (35%). In conclusion, the BAM mixture demonstrated high antibacterial efficacy and holds promise as a natural agent for controlling multidrug-resistant (MDR) P. aeruginosa strains in the future.