Metabolic Adaptations: Comparison of Amino Acid Profiles of SK-MEL-30 Melanoma Cells and CCD-1135Sk Fibroblasts

This study provides original data on metabolic reprogramming of tumor cells by comparing amino acid profiles between malignant melanoma cells and healthy human dermal fibroblast cells. Quantitative amino acid analyses performed on SKMEL-30 (melanoma) and CCD-1135Sk (normal fibroblast) cells using LC-MS/MS technology showed that the total amino acid load in melanoma cells was approximately 80% higher than in normal fibroblasts (7595.94 ng/mL vs. 4225.35 ng/mL). Significant increases were detected in amino acids such as glutamate, valine, leucine, isoleucine, serine, alanine, threonine, and glycine. These increases support processes such as rapid proliferation of tumor cells, meeting energy needs, maintaining redox balance, and stress response. In addition, the dramatic increase in metabolites such as taurine and GABA in melanoma cells suggests that these cells gain a microsurvival advantage. The findings reveal that melanoma-specific amino acid metabolism has significant potential for biomarker development and targeted metabolic treatment approaches.