From monolayer to spheroid: assessing influenza a virus infection in 2D and 3D cell culture of A549 and HEK293
We conclude that Alg + MC matrix, with its increased porosity and lower cohesion compared to Alg alone, was easier to dissolve but more difficult to re-solidify. One possible explanation for the observed higher viral replication in this matrix is that it may have facilitated improved viral access to the cells. Future modifications that increase virus-cell interaction time in this system could further enhance infection efficiency.
Authors
- Parvaneh Mehrbod (ORCID: https://orcid.org/0000-0002-8391-9228)
- Hadiseh Shokouhi (ORCID: https://orcid.org/0000-0002-4325-6554)
- Mahsa Mollapour Sisakht (ORCID: https://orcid.org/0000-0003-0396-676X)
- Fatemeh Gholizadeh (ORCID: https://orcid.org/0000-0002-1518-4629)
- Hosnieh Sokhandan
Institutions
- Pasteur Institute of Iran (IR)
- Shiraz University of Medical Sciences (IR)
- Farabi Hospital (IR)
- Tehran University of Medical Sciences (IR)
Publication Details
- Journal
- Virology Journal
- Published
- 2025-09-29
- DOI
- https://doi.org/10.1186/s12985-025-02939-9
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Tehran University of Medical Sciences and Health Services
- Pasteur Institute of Iran