STALARD: Selective Target Amplification for Low-Abundance RNA Detection
STALARD provides a sensitive, simple, and accessible method for isoform-level quantification of low-abundance transcripts that share a known 5'-end sequence. Its compatibility with both qPCR and long-read sequencing makes it a versatile tool for analyzing transcript variants and identifying previously uncharacterized 3'-end structures, provided that isoform-specific 5'-end sequences are known in advance.
Authors
- Daesong Jeong (ORCID: https://orcid.org/0000-0003-0224-1687)
- Chulmin Park (ORCID: https://orcid.org/0000-0001-9147-0478)
- Ilha Lee
Institutions
- Seoul National University (KR)
- National University College (PR)
- Institute of Molecular Biology (AM)
Publication Details
- Journal
- Plant Methods
- Published
- 2025-09-29
- DOI
- https://doi.org/10.1186/s13007-025-01443-z
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea