Clinical value of abnormal expression of serum lncRNA HOTTIP rs1859168 in recurrent spontaneous abortion

Abstract Objectives The objective of this research was to investigate the correlation between HOTTIP polymorphisms and recurrent spontaneous abortion (RSA) susceptibility. Methods This study included 265 RSA patients as the case group and 260 healthy volunteers as the control group. Genotyping was performed using the TaqMan probe-based polymerase chain reaction (PCR) method. Serum HOTTIP expression in subjects was detected using reverse transcription quantitative polymerase chain reaction (RT-qPCR). The clinical diagnostic value of HOTTIP for RSA was evaluated by a receiver operating characteristic (ROC) curve. Results The frequency of C alleles in the case group was lower than that of the control group, and the frequency of A alleles was higher than that of the control group, and the A allele was a risk factor for the onset of RSA (p<0.05). The rs1859168 gene polymorphism was correlated with RSA susceptibility under the codominant, dominant, and recessive inheritance models (p<0.05). Serum HOTTIP expression was elevated in RSA patients. The ROC curve showed that serum HOTTIP had good diagnostic value for RSA, with an area under the curve (AUC) of 0.903, a sensitivity of 87.5 % and a specificity of 81.2 %. Conclusions HOTTIP rs1859168 gene polymorphism is associated with RSA susceptibility. People bearing the AC and AA genotypes have a higher risk of RSA.