Rekabetçi Alel-Spesifik PCR (KASP) İşaretleyicileri Kullanılarak Buğday Yerel Bitki Germ-Plazmasının Tarımsal Hastalıklara Duyarlılık ve Kalite Özellikleri Açısından Değerlendirilmesi

In the study, the genetic variation in agronomic, disease resistance and quality traits of seventy-three wheat landraces from Pakistan was analyzed by using 65 Kompetitive Allele-Specific PCR (KASP) assays. This research was intended to underpin the alleles for economically important genes in wheat landraces. Alleles, Rht-B1a and Rht-B1b were found in 64% and 30% of the population; while Rht-D1a and Rht-D1b were identified in 90% and 10% of the population respectively. Alleles responsible for flowering time Ppd-B1 were detected as sensitive in 46%, and insensitive in 44% of the population while 56 % and 39% insertion and deletions spanning 2Kb distance were observed in Ppd-D1. Similarly, 8, 15 and 2 assays were performed to study the genetic variation in vernalization, yield and drought tolerance. About 18% of the landraces were found with TaDREB_B1a allele which is drought tolerant. Unfortunately, more than eighty percent of wheat landraces were found to be susceptible to leaf rust confirmed with six assays. In addition, more than 90% of landraces were susceptible to stripe rust, stem rust and Fusarium head blight. Identified novel alleles from the current panel of wheat landraces can be used for marker-assisted as well genomic selection by breeding programs.