<i>In operando</i> assessment of H<sub>2</sub>O<sub>2</sub> production by a multicopper oxidase under direct electron transfer with a gold electrode
A deeper understanding of the intricate redox processes at biotic/abiotic interfaces requires advanced analytical tools. We present the localized and real-time characterization of a multicopper oxidase adsorbed on a gold electrode using a dedicated microbiosensor, providing detailed and spatially resolved information. Enzymatic O<sub>2</sub> reduction is shown to proceed without detectable H<sub>2</sub>O<sub>2</sub> accumulation. Instead, H<sub>2</sub>O<sub>2</sub> originates from direct O<sub>2</sub> conversion at the gold surface.
Authors
- Wolfgang W. Schuhmann (ORCID: https://orcid.org/0000-0003-2916-5223)
- Lı́gia O. Martins (ORCID: https://orcid.org/0000-0003-0082-9591)
- Felipe Conzuelo (ORCID: https://orcid.org/0000-0002-3316-2642)
- Vânia Brissos (ORCID: https://orcid.org/0000-0002-8171-0113)
- Panpan Wang (ORCID: https://orcid.org/0000-0002-9171-9103)
Institutions
- Universidade Nova de Lisboa (PT)
- Ruhr University Bochum (DE)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc03482e
- Primary Topic
- Electrochemical sensors and biosensors
- Type
- article
- Field-Weighted Citation Impact
- 0.00