Protocol for trapping transient endogenous formaldehyde in live cells to visualize its signaling

Formaldehyde is emerging as a key signaling modulator. Here, we present Trami, a protocol that traps endogenous formaldehyde in live cells and converts its transient signals into stable proximal protein labels for subsequent signaling study. We describe probe design, live-cell administration, and imaging procedures for signaling deconvolution. Trami offers a powerful tool for elucidating formaldehyde-mediated signaling pathways. For complete details on the use and execution of this protocol, please refer to Pan et al.¹.