AAV2retro Enters Axons of Passage and Extensively Transduces Corticospinal Neurons After Injection into Spinal White Matter
We conclude that AAV2retro can enter axons of passage in the dorsal column white matter of the spinal cord, and that injecting the cervical dorsal columns can efficiently target both forelimb and hindlimb corticospinal neurons in mice. This new approach for targeted gene delivery to corticospinal neurons could improve the safety and specificity of regenerative gene therapies for spinal cord injury.
Authors
- Michael J. Castle (ORCID: https://orcid.org/0000-0001-7566-6454)
- Keigo Nakashima (ORCID: https://orcid.org/0000-0002-3053-3014)
- Shanshan Wang (ORCID: https://orcid.org/0000-0001-8249-4291)
Institutions
- University of California San Diego (US)
Publication Details
- Journal
- Brain Sciences
- Published
- 2025-09-28
- DOI
- https://doi.org/10.3390/brainsci15101058
- Primary Topic
- Nerve injury and regeneration
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- University of California, San Diego