Manufacturing of Zinc Oxide Nanoparticles for Medical Applications

The pulsed laser ablation in liquid (PLAL) method was used to synthesize ZnO NPs, which were subsequently characterized for their structural, optical, and antibacterial properties.FE-SEM showed the nanoparticles with needle morphology, of size 20-50 nm, which was also confirmed by XRD analysis with high crystallinity of 28.09-31.86nm for average crystallite size.The absorption band at 284.5 nm revealed in UV-Vis spectroscopy was characteristic of the use of ZnO NPs.Antibacterial activity testing was assessed by the well diffusion assay in which inhibition zones of up to 25 1.2 mm for S. aureus and 20 1.5 mm for E. coli were seen at 50 L (50 mg/10 mL) concentration.Results demonstrate that ZnO NPs can act as effective antibacterial agents by damaging bacterial membranes.Compared to the previous studies, which focused on spherical shapes or chemically synthesized ZnO NPs, however, this work can show the macroporous fabrication of highpurity, needle-shaped ZnO NPs with a single step, eco-friendly pulsed laser ablation in liquid technique, leading to higher antibacterial activity by enhancing surface area reactivity and crystallinity.The results of this study indicate that the pulsed laser ablation in liquid method is a suitable way to produce high-purity ZnO NPs for potential use in biomedical fields, including antimicrobial and wound healing applications.