Prokaryotic Expression of GP5 Protein of Betaarterivirus suid 2 and its Applications as Coating Antigen in Development of Indirect ELISA

Background: Porcine reproductive and respiratory syndrome (PRRS) is an emerging swine disease, causing huge economic losses. This virus has four immunogenic proteins out of which glycoprotein 5 (GP5) is the major immunogenic protein, followed by nucleocapsid protein (N), glycoprotein 3 (GP3) and matrix protein (M). The present study was designed to express the major immunogenic protein (GP5) of an isolate of Betaarterivirus suid 2 in the prokaryotic system and its application as a coating antigen for ELISA. Methods: GP5 protein was expressed in the prokaryotic system and the expression was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. The GP5 protein was purified by nickel affinity chromatography. The purified GP5 protein was used as coating antigen in indirect enzyme-linked immunosorbent assay (ELISA) and was compared with the commercial kit PRRSV Antibodies ELISA Kit (Cat no.: E-AD-E006, Elabscience). Result: The indirect ELISA results utilizing the present assay and the commercial kit were comparable with accurate detection of positive and negative serum samples.