Synthesis, duplex-forming properties, and enzymatic resistance of oligonucleotides containing <i>N</i> -acylsulfonamide linkages
We report the synthesis of (2-(<i>N</i>-acetylsulfamoyl)ethyl)carbamate (NAC)-linked thymidine dinucleotide <i>via</i> the sulfo-click reaction and its incorporation into DNA oligonucleotides. Thermal denaturation studies indicate modest destabilization at duplex ends (∼1 °C) and stronger effects in the middle (∼7 °C), while the NAC linkage provides complete resistance to nucleases.
Authors
- Romain Amador (ORCID: https://orcid.org/0000-0002-7498-0334)
- Michaël Smietana (ORCID: https://orcid.org/0000-0001-8132-7221)
- Guillaume Clavé (ORCID: https://orcid.org/0000-0001-6401-0935)
- Carine Baraguey
Institutions
- École Nationale Supérieure de Chimie de Montpellier (FR)
- Centre National de la Recherche Scientifique (FR)
- Université de Montpellier (FR)
- Institut des Biomolécules Max Mousseron (FR)
Publication Details
- Journal
- Organic & Biomolecular Chemistry
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5ob01382h
- Primary Topic
- DNA and Nucleic Acid Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Agence Nationale de la Recherche