Regional Conservation and Transcriptional Regulation of Tumor-Associated Genes by macroH2A1 Deposition in Mammalian Cells

Histone variant macroH2A1 (mH2A1) has been widely recognized as a suppressor of gene expression. Recently, a cell cycle-dependent deposition of mH2A1 was discovered in mouse cells, but whether this process exists in human chromatin is unclear, which might be crucial for related diseases, particularly cancer. In this study, with native chromatin immunoprecipitation (nChIP-seq), we firstly demonstrate that dynamic mH2A1 domains occur in both normal and cancerous human cells and have conserved enrichment patterns across species. Our findings further provide new epigenetic insights into the role of mH2A1 in malignant proliferation, offering a novel perspective for future cancer research.