Development of a single-tube, dual-target CRISPR Cas12a/Cas13a system for rapid screening of coinfection with respiratory syncytial virus and rhinovirus
The RT-RPA-CRISPR-Cas12a/13a system provides a rapid, sensitive, and efficient solution for RSV and HRV coinfection detection. This method supports early diagnosis and improved clinical management, offering significant potential for public health applications in preventing severe respiratory complications in children.
Authors
- Zihan Zhou (ORCID: https://orcid.org/0000-0002-8524-1000)
- Cheng Guo (ORCID: https://orcid.org/0000-0001-7489-7381)
- Minghui Ji (ORCID: https://orcid.org/0000-0003-3399-9933)
- Wenwu Zhao (ORCID: https://orcid.org/0000-0001-5342-354X)
- Xinyi Zhao (ORCID: https://orcid.org/0000-0002-9766-8925)
- Tianchi Zhuang
- Chang Gao
Institutions
- Sun Yat-sen University (CN)
- Second Affiliated Hospital of Nanjing Medical University (CN)
- Nanjing Medical University (CN)
Publication Details
- Journal
- Virology Journal
- Published
- 2025-09-29
- DOI
- https://doi.org/10.1186/s12985-025-02938-w
- Citations
- 2
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 2.71
Funders
- Jiangsu Commission of Health
- Jiangsu Provincial Department of Education