ANTIOXIDANT, ANTIARTHRITIC, AND ANTIMICROBIAL ACTIVITY OF SYNTHESIZED SILVER NANOPARTICLES USING ANNONA SQUAMOSA ROOT UNDER IN-VITRO STUDY

Biosynthesized silver nanoparticles from ethanolic extract of root of Annona squamosa shows the strong antioxidant and antiarthritic activities.The characterization of synthesized silver nanoparticles shows the shape, size, particle range by FTIR, UV-Visible, SEM, XRD and EDAX analysis.SEM analysis shows the morphology of silver Nanoparticle.The silver nanoparticles lie between 74.08 to 134.2nm region and the average size of silver nanoparticles is 455.9nm which shows the shape of silver nanoparticles as spherical.The antioxidant activity of standard drug ascorbic acid shows maximum inhibition of 82.17% at 100g/ml and ethanolic extract of synthesized silver nanoparticles shows the maximum inhibition at 100g/ml is 67.33%.The antiarthritic activity of synthesized silver nanoparticles shows maximum inhibition of 72.07% in protein denaturation and for standard diclofenac sodium shows maximum inhibition of 81.98% at 100g/ml.The HRBC method shows the maximum inhibition 68.10% for synthesized silver nanoparticles and 84.49% for standard diclofenac sodium at 100g/ml.The antimicrobial activity was done against three bacterial and two fungal species which includes Escherichia coli, Klebsiella pneumonia, Enterococcus faecalis, Staphylococcus aureus and Candida albicans, Candida vulgaris.Among these species, Escherichia coli had a maximum zone of inhibition of 8mm at 100g/ml concentration.