The Inhibitory Effects of Resveratrol on Tumor Growth and Metastasis in Esophageal Adenocarcinoma

Objective: Resveratrol is a small polyphenol that has been extensively researched across various therapeutic areas. Its inhibitory effect on esophageal cancer cell growth has also been demonstrated in a dose- and time-dependent manner. However, the underlying mechanism of this effect has not been fully elucidated yet. We aimed to investigate the anti-proliferative and anti-metastatic effects of resveratrol on esophageal adenocarcinoma (EAC) cells in vitro. Methods: The EAC cells were treated with various doses of resveratrol, and cell viability was determined using the WST-1 assay. The apoptosis rate and the level of intracellular reactive oxygen species (ROS) were measured using flow cytometry. Adhesion, wound healing, and colony formation assays were carried out to evaluate the anti-metastatic effects of resveratrol. Results: Resveratrol decreased the cell viability of OE33 cells in a dose-dependent manner. 150 µM resveratrol reduced cell viability by 49% compared to the control by increasing ROS levels. This inhibition was associated with the induction of apoptosis. The analysis revealed that resveratrol also suppressed the adhesion, migration, and colony forming capacity of the cells. Conclusion: Resveratrol lower the viability and metastatic potential of EAC cells. These findings suggest resveratrol may be further evaluated clinically for this malignancy in humans.