LC-MS/MS-based discovery of new cytochalasin from fungus <i>Rosellinia sanctae-cruciana</i>
The ethyl acetate extract of the fungus <i>Rosellinia sanctae-cruciana</i> was analysed using LC-MS/MS, revealing a peak at <i>m/z</i> 558.272 [M + H]<sup>+</sup> with the molecular formula C<sub>30</sub>H<sub>39</sub>NO<sub>9</sub>, indicative of the cytochalasin class. The isolated compound, 18 Da higher in molecular weight than 19,20-Epoxycytochalasin-N, featured an opened diol structure at positions 5 and 6. Named Cytochalasin-Augusta, it was purified using HPLC and characterised by <sup>1</sup>H NMR,<sup>13</sup>C NMR, HSQC, HMBC, and NOESY spectroscopy.
Authors
- Chetan Kumar
- Nisha Sharma (ORCID: https://orcid.org/0000-0001-7674-5007)
- Poonam Choudhary
- Manoj Kushwaha (ORCID: https://orcid.org/0000-0003-3133-5762)
- Sundeep Jaglan (ORCID: https://orcid.org/0000-0002-5691-7980)
- Mohd Murtaza (ORCID: https://orcid.org/0000-0001-9512-3772)
- Vidushi Abrol (ORCID: https://orcid.org/0000-0002-8607-0282)
- Priya Kumari (ORCID: https://orcid.org/0009-0001-7637-440X)
- Rohit Singh
Institutions
- Lovely Professional University (IN)
- MIT World Peace University (IN)
- Indian Institute of Integrative Medicine (IN)
- Dehradun Institute of Technology University (IN)
- Academy of Scientific and Innovative Research (IN)
Publication Details
- Journal
- Natural Product Research
- Published
- 2025-09-29
- DOI
- https://doi.org/10.1080/14786419.2025.2565279
- Primary Topic
- Plant and Fungal Interactions Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00