Transcriptomic insights and feeder-free culturing of porcine expanded potential stem cells from cloned embryos
Our modified culture medium combined with the 2× Matrigel coating system facilitated the transition to feeder-independent culture conditions. These findings facilitate the establishment of a feeder-free culture system while preserving pluripotency and differentiation potential.
Authors
- Hyerin Choi (ORCID: https://orcid.org/0000-0001-6320-456X)
- Ha‐Neul Kim (ORCID: https://orcid.org/0000-0002-1430-1117)
- Lian Cai (ORCID: https://orcid.org/0000-0002-1096-700X)
- Mirae Kim
- Sang‐Hwan Hyun (ORCID: https://orcid.org/0000-0001-6011-2555)
- Eunhye Kim (ORCID: https://orcid.org/0000-0001-5102-6794)
Institutions
- Chungbuk National University (KR)
- Gyeongsang National University (KR)
- Chungbuk National University Hospital (KR)
- University at Buffalo, State University of New York (US)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-09-29
- DOI
- https://doi.org/10.1186/s13287-025-04627-5
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00