Laparoscopic near-infrared hyperspectral imaging system for identifying living porcine nerves and unexposed arteries

Surgical resection remains a key curative option for cancer, with minimally invasive approaches increasingly adopted. To enhance intraoperative visualization, we developed a laparoscopic near-infrared hyperspectral imaging (NIR-HSI) system comprising a custom laparoscope, supercontinuum light source, and acousto-optic tunable filter. Ex vivo NIR-HSI of porcine arteries, mesentery, and nerves revealed distinct spectral signatures from 1000-1402 nm. Pixel-based classification via neural networks achieved >99% accuracy, sensitivity, and specificity in most cases. In vivo imaging of a living pig enabled identification of exposed nerves (88.4% accuracy, 68.7% recall) and unexposed arteries (83.2% accuracy, 60.2% recall). These results demonstrate that laparoscopic NIR-HSI can differentiate tissues with similar coloration and detect structures embedded beneath the surface, offering potential for safer minimally invasive surgeries.

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Publication Details

Journal
Biomedical Optics Express
Published
2025-09-29
DOI
https://doi.org/10.1364/boe.576004
Primary Topic
Infrared Thermography in Medicine
Type
article
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article

Laparoscopic near-infrared hyperspectral imaging system for identifying living porcine nerves and unexposed arteries

Nobuyoshi Takeshita, Abián Hernández-Guedes, Hideo Yokota, Hiroshi Takemura et al.
Biomedical Optics Express
Infrared Thermography in Medicine
article

Laparoscopic near-infrared hyperspectral imaging system for identifying living porcine nerves and unexposed arteries

Nobuyoshi Takeshita, Abián Hernández-Guedes, Hideo Yokota, Hiroshi Takemura, Hiro Hasegawa, Gustavo M. Callicó, Kohei Soga, Seiya Hayashi, Toshihiro Takamatsu, Nariaki Okamoto, Shintaro Arakaki
article en

Abstract

Surgical resection remains a key curative option for cancer, with minimally invasive approaches increasingly adopted. To enhance intraoperative visualization, we developed a laparoscopic near-infrared hyperspectral imaging (NIR-HSI) system comprising a custom laparoscope, supercontinuum light source, and acousto-optic tunable filter. Ex vivo NIR-HSI of porcine arteries, mesentery, and nerves revealed distinct spectral signatures from 1000-1402 nm. Pixel-based classification via neural networks achieved >99% accuracy, sensitivity, and specificity in most cases. In vivo imaging of a living pig enabled identification of exposed nerves (88.4% accuracy, 68.7% recall) and unexposed arteries (83.2% accuracy, 60.2% recall). These results demonstrate that laparoscopic NIR-HSI can differentiate tissues with similar coloration and detect structures embedded beneath the surface, offering potential for safer minimally invasive surgeries.

Biomedical Optics ExpressVol. 16(11)
Universidad de Las Palmas de Gran Canaria (ES), Tokyo University of Science (JP), University of the Ryukyus (JP), National Cancer Center Hospital East (JP), University of the Ryukyus University Hospital (JP), RIKEN Center for Advanced Photonics (JP), National Institute of Advanced Industrial Science and Technology (JP)
Openalex Percentile: Top 60%
Infrared Thermography in Medicine
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