Protocol for immunofluorescence imaging of epitope-tagged protein in Arabidopsis shoot apical meristem
Proteins expressed in or translocated to the shoot apical meristem (SAM) often play pivotal roles in regulating plant development. Here, we present a step-by-step protocol for immunofluorescence imaging to analyze epitope (FLAG)-tagged proteins in the Arabidopsis SAM. This technique may be suitable to visualize mobile proteins because small epitope tags are less disruptive than a relatively large fluorescent protein fusion. For complete details on the use and execution of this protocol, please refer to Takagi et al.<sup>1</sup>.
Authors
- Takato Imaizumi (ORCID: https://orcid.org/0000-0001-9396-4412)
- Naoyuki Uchida (ORCID: https://orcid.org/0000-0002-4123-6154)
- Hiroshi Takagi (ORCID: https://orcid.org/0000-0003-0321-0284)
Institutions
- University of Washington (US)
- University of Washington Applied Physics Laboratory (US)
- Nagoya University (JP)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-09-27
- DOI
- https://doi.org/10.1016/j.xpro.2025.104112
- Primary Topic
- Plant tissue culture and regeneration
- Type
- article
- Field-Weighted Citation Impact
- 0.00