EVALUATION OF THE ANTIBACTERIAL ACTIVITIES OF NATURAL COUMARINS FROM APIACEAE PLANTS

Objective: This study aimed to determine the antibacterial activity of fourteen natural coumarin derivatives against various bacterial strains including Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 6538), Staphylococcus epidermidis (ATCC 12228), Escherichia coli (ATCC 8739), and Salmonella enterica serovar Typhimurium (ATCC 14028). Material and Method: The antibacterial activity of the following fourteen natural coumarins: 4’-senecioyloxyostol (1), deltoin (2), smyrnioridin (3), karatavicinol (4), badrakemin (5), colladonin (6), badrakemone (7), colladonin acetate (8), anatolicin (9), 14’-hydroxycolladonin (10), 14’-hydroxybadrakemin (11), 14’-acetoxycolladonin (12), 14’-acetoxybadrakemone (13), and 14’-acetoxybadrakemin (14) was investigated by the disc diffusion method. Result and Discussion: Among the microorganisms tested, Staphylococcus epidermidis showed the highest sensitivity to coumarin derivatives, while Escherichia coli was the least sensitive. Colladonin and colladonin acetate showed activity against all bacterial strains tested, except E. coli and S. enterica serovar Typhimurium, respectively. In particular, 4’-senecioyloxyostol exhibited the strongest antibacterial activity at a concentration of 64 µg/disc, with zones of inhibition ranging from 6.6 to 7.5 mm.