Localization of single molecules with structured illumination and structured detection
A new high-precision single-molecule localization scheme, ISM-FLUX, is an implementation of MINFLUX using image-scanning microscopy (ISM) with a single-photon avalanche diode (SPAD) array detector. ISM-FLUX results in a larger localization range, enhancing the robustness of the localization scheme and it also potentially enables experiments in which absorption and emission of a single fluorophore can be probed independently.
Authors
- Luciano A. Masullo (ORCID: https://orcid.org/0000-0001-8664-5448)
Institutions
- Max Planck Institute of Psychiatry (DE)
- Max Planck Institute of Biochemistry (DE)
Publication Details
- Journal
- Light Science & Applications
- Published
- 2025-09-29
- DOI
- https://doi.org/10.1038/s41377-025-01980-1
- Citations
- 1
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.63