Complete Genome Sequence of <i>Komagataeibacter saccharivorans</i> KS1 Isolated from a Kombucha SCOBY

Kombucha is a traditional tea-based fermented beverage fermented by a symbiotic culture of bacteria and yeast (SCOBY) [1].The members of Komagataeibacter, belonging to the family Acetobacteraceae, are aerobic Gram-negative bacteria frequently found in SCOBY; they are well known for their roles in cellulose synthesis and acid production [2].Studies have focused on microbial strains that can degrade caffeine, a bioactive compound found in tea and coffee.Such microorganisms show potential for the development of low-caffeine functional beverages and the bioremediation of caffeinecontaminated environments [3,10].In this study, we reported the complete genome sequence of Komagataeibacter saccharivorans KS1 isolated from a kombucha SCOBY and described its genetic potential for caffeine metabolism.K. saccharivorans KS1 was isolated from a commercial kombucha SCOBY.Its colonies were obtained by streak-Komagataeibacter saccharivorans KS1 was isolated from a kombucha SCOBY.Whole-genome sequencing revealed a 4,036,451 bp circular chromosome with a GC content of 58.8%.Genome annotation predicted 3,947 protein-coding sequences, 59 tRNAs, and 15 rRNAs.Functional annotation identified six methylxanthine demethylase genes and two oxidoreductase genes involved in caffeine metabolism.This study provided insights into the genomic characteristics of K. saccharivorans KS1 and supported its potential as a promising candidate for caffeine degradation.It could be applied to low-caffeine fermented products and environmental biotechnology.