Cytotoxicity, Acute and Subacute Toxicity Evaluation of Hydroethanolic Extract of <i>Dialium Guineense </i>Willd Fruit Pulp

<i>Objective: </i>This study aims to evaluate the acute and subacute toxicity, as well as the cytotoxicity of the hydroethanolic extract of <i>D. guineense</i> willd fruit pulp. <i>Methodology: </i>Three different tests were performed. The cytotoxicity test was performed using <i>Artemia salina</i> larvae. The acute toxicity test was conducted by single oral administration of <i>Dialium guineense </i>at 5000 mg/kg. When subacute toxicity tests were performed by repeated oral administration of <i>Dialium guineense</i> at 250, 500, and 1000 mg/kg/day for 28 days. <i>Results: </i>The fruit pulp <i>of Dialium guineense</i> presented a CL<sub>50</sub> equal to 1.40 mg/mL and an LD<sub>50</sub> greater than 5000 mg/kg. This pulp at the dose of 1000 mg/kg significantly reduced blood glucose as well as the overall white blood cell counts, particularly neutrophils and eosinophil polymorphonuclear cells, at low doses after 28 days of treatment. <i>Conclusion: </i>The hydroethanolic extract of the <i>Dialium guineense </i>willd fruit pulp does not show signs of cytotoxicity, major acute or subacute toxicity.