Effect of combination of autologous platelet-rich plasma and nano fat application on donor site of split thickness graft on scar appearance.

Background: Nano fat grafting is the procedure of converting adipose tissue into liquid fat and obtaining adipose stem cells and stromal vascular fraction cells. Platelet-rich plasma (PRP) stimulates growth factors and starts signaling pathways that promote tissue repair and regeneration. The primary objective of this research was to assess the effectiveness of combining autologous platelet-rich plasma (PRP) with nanofat grafting application on the donor site of split-thickness grafts.Methods: This is a prospective interventional study; including 30 patients of both genders undergoing medium split-thickness skin grafting. The resultant area at the donor site was used as the test area. It was divided into equal halves; one was covered by our combination autologous PRP and nano fat (1:1) solution (part A), while the other half was covered by Vaseline gauze (part B).Results: The mean age among examined patients was 36.03 ± 17.11 years. Part A had significantly shorter healing time compared to part B. Part A had significantly lower incidences of infection and itching when compared to part B. While no significant difference was reported between parts A and part B regarding incidences of pain or hypertrophic scar.Conclusion: The application of a combination of autologous platelet-rich plasma and nano fat on the donor site of a split-thickness graft shortened the time needed for epithelialization and healing, lowered the occurrence of infection at the donor site of STSG, decreased the symptoms associated with the scar, and slightly improved the quality of the scar.