Physiological and biochemical evaluation of Vitamin H7 in Rabbits stressed by Triton

Vitamin H7 (biotin) is essential for fatty acid synthesis and glucose metabolism. Under stress conditions, such as those induced by Triton (a nonionic surfactant that increases oxidative stress markers and induces hyperlipidemia), the body may require more biotin to manage oxidative stress and maintain metabolic balance. Studying its effects in Triton-stressed rabbits can reveal its potential protective benefits. The aim was to understand how Triton affects the vital functions of rabbits and whether Vitamin H7 plays a role in protecting these functions from negative impacts. Forty male rabbits were divided into four groups and treated daily for eight weeks. The control received no treatment. The Triton group was given water with Triton 300 mg/kg. The Vitamin H7 group received 200 µg/kg, and the Triton and Vitamin H7 group received both treatments. The treatment continued for eight consecutive weeks. The Triton group showed the highest activity of aryl esterase and bilirubin, as well as the lowest activity of superoxide dismutase (SOD) and acetylcholinesterase (Ach), while the Vitamin H7 group showed low activity of aryl esterase and Total Bilirubin, Along with high superoxide dismutase (SOD) activity. The Triton with Vitamin H7 group showed mitigation of the negative effect of triton and improvement in total Bilirubin levels. Triton induces toxic effects, such as oxidative stress, and impacts cell membranes, leading to an increase in aryl esterase activity and a decrease in Ach and SOD. In contrast, Vitamin H7 helps mitigate these toxic effects by supporting cellular metabolism and improving enzyme function, reducing total bilirubin levels, and enhancing liver function.