Isolation of cis-2-methylpalmitolein-9 acid from the fungus <i>Trichoderma asperellum Uz-A4</i> and its Spectroscopic analysis

Today, the use of natural and non-harmful substances is one of the main and a pressing issue in all industries. In this regard, most researchers are focused on extracting the necessary components from natural sources and raw materials. At the same time, the purpose of the present practical work is based on the extraction of biologically active substances from microbodies (fungi). In this exploratory study, the extraction of secondary metabolites from the strain Trichoderma asperellum Uz-A4 was performed. Fungal biomass was prepared and extracted using alcoholic extraction, and the resulting extract was fractionated by column chromatography using various solvents. In the chloroform: methanol 9:1 system step of the fractionation process, a high content of cis-2-methylpalmitolein-9 acid was formed, along with a number of secondary metabolites. For the first time, this unsaturated fatty acid was isolated from the strain of Trichodema asperellum Uz-A4. When the structure of this substance was isolated as a pure substance and its structure was studied by other spectroscopic assays, it turned out to be cis-2-methylpalmitoleic acid.