Extraction Technique Impacts on Phenolic Profile, Antioxidant Capacity, and Enzyme Inhibition by <i>Anthemis pseudocotula</i>

Abstract Plant‐derived phenolic and flavonoid compounds exhibit significant health benefits, encompassing antioxidant and enzyme inhibitory activities. We compared three extraction techniques—maceration (MAC‐ME), Soxhlet extraction (SOE‐ME), and ultrasound‐assisted extraction (UAE‐ME)—for their effectiveness in recovering bioactive compounds from Anthemis pseudocotula and assessed the resulting antioxidant and enzyme inhibition profiles. MAC‐ME and UAE‐ME produced higher total phenolic contents than SOE‐ME, with MAC‐ME achieving the greatest yield. In antioxidant assays, MAC‐ME showed superior performance in the phosphomolybdenum and ferrous ion chelating tests. Conversely, UAE‐ME delivered the strongest reducing power in the CUPRAC and FRAP assays, and both MAC‐ME and UAE‐ME outperformed SOE‐ME in ABTS radical scavenging. Regarding enzyme inhibition, MAC‐ME and UAE‐ME exhibited more potent acetylcholinesterase (AChE) inhibition than SOE‐ME; SOE‐ME was most effective against tyrosinase, whereas MAC‐ME showed the highest α‐amylase inhibition. Overall, MAC‐ME proved the most effective for extracting phenolic and flavonoid compounds from A. pseudocotula , with UAE‐ME also demonstrating notable antioxidant efficacy, underscoring the impact of extraction technique on bioactivity.