Dendrimer-based DNA biosensor for HIV virus detection

In this work, we propose an innovative electrochemical DNA biosensor for the early, rapid, selective, and sensitive detection of the human immunodeficiency virus (HIV) based on its genetic code. This platform is based on the integration of few-layer bismuthene (FLB) which provides a nanostructured surface and facilitates the anchoring of the DNA dendrimer which incorporates the biorecognition element (a capture probe complementary to the HIV-specific DNA sequence). The hybridization between the capture probe and the specific HIV target sequence is detected using Azure A (AA) as a redox indicator. The dendrimer-based DNA biosensor selectively detects HIV from 10.0 fM to 10.0 pM, exhibit a low detection limit of 3.03 fM and a 60-day stability, and has been validated using plasma samples from infected patients with varying viral loads.

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Publication Details

Journal
Biosensors and Bioelectronics
Published
2025-09-27
DOI
https://doi.org/10.1016/j.bios.2025.118014
Primary Topic
Advanced biosensing and bioanalysis techniques
Type
article
Field-Weighted Citation Impact
0.00

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article

Dendrimer-based DNA biosensor for HIV virus detection

Tania García‐Mendiola, Estefanía Enebral-Romero, Brais González-Tobío, Marisa Montes et al.
Biosensors and Bioelectronics
Advanced biosensing and bioanalysis techniques
article

Dendrimer-based DNA biosensor for HIV virus detection

Tania García‐Mendiola, Estefanía Enebral-Romero, Brais González-Tobío, Marisa Montes, Félix Zamora, Iker Falces‐Romero, Mónica Luna, David López-Diego, Marta Toldos-Torres, Marta Failde
article en

Abstract

In this work, we propose an innovative electrochemical DNA biosensor for the early, rapid, selective, and sensitive detection of the human immunodeficiency virus (HIV) based on its genetic code. This platform is based on the integration of few-layer bismuthene (FLB) which provides a nanostructured surface and facilitates the anchoring of the DNA dendrimer which incorporates the biorecognition element (a capture probe complementary to the HIV-specific DNA sequence). The hybridization between the capture probe and the specific HIV target sequence is detected using Azure A (AA) as a redox indicator. The dendrimer-based DNA biosensor selectively detects HIV from 10.0 fM to 10.0 pM, exhibit a low detection limit of 3.03 fM and a 60-day stability, and has been validated using plasma samples from infected patients with varying viral loads.

Biosensors and BioelectronicsVol. 290
Madrid Institute for Advanced Studies (ES), Hospital Universitario La Paz (ES), Hospital La Paz Institute for Health Research (ES), Institute of Micro and Nanotechnology (ES), Centro de Biología Molecular Severo Ochoa (ES), Universidad Autónoma de Madrid (ES)
Comunidad de Madrid, Ministerio de Ciencia, Innovación y Universidades, European Commission, Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación, HORIZON EUROPE European Innovation Council
Openalex Percentile: Top 77%
Advanced biosensing and bioanalysis techniques
DOIOpen Access PDFOpenAlex
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