Antibacterial Efficiency of the Extracts of Euphorbia condylocarpa TuberRoots and Leaves on Three Multidrug Resistance Bacterial Isolates usingGreen Synthesis of Zinc Oxide Nanoparticle Techniques

The perform study was executed to inspect the phytochemical complement, antimicrobial activity of chloroform (ChL),ethyl acetate (EA), as well as ethanol (ET) extracts in the leaves and root tubers of spurge E. condylocarpa along withgreen synthesis, characterization and antibacterial attain of zinc oxide nanoparticles on multidrug-resistant bacterial isolatessuch as staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli by Minimum Inhibitory Concentrations(MIC). The inclined ZnO NPs were categorized along UV-vis, FT-IR, SEM, XRD, together with EDX. This experimentapplied in the laboratories of Biology Department, College of Science Salahaddin University, and conducted according toa completely randomized design (CRD) with three replications. E.condylocarpa leaves and root tubers have been retaineras reducing, capping and stabilizing agents, the configuration of ZnO NPs portrait by shift in color from dark yellow toyellowish white. X-ray diffraction (XRD) valid the crystallinity of ZnO NPs. FT-IR spectroscopy corroborated thefunctional groups of leaves and root tubers extracts Qualitative phytochemicals analysis focuses on the presence or absentof several chemical groups in the plant parts. Total condensed tannin, and total phenol was done in both parts. The resultsshowed that the amount of total condensed tannin was more in leaves as comparison to root tubers, while total phenoliccontent in ethanol extract more than in ethyl acetate then in chloroform in both leaves and root tubers. The crude extractsof leaves and root tubers showed that the plant contains different phytochemicals such as terpenoids, steroids, saponin,flavonoids, phenol, tannin, coumarins, quinone, and alkaloids. The lowest significant MIC values with E. coli was 4.17mg/ml for ethanol leaf extract of E. condylocarpa.