Formulation, Development, Characterization of Tablet Containing Glucosamine Sulphate from Agaricus bisporus

The present study aimed to synthesize glucosamine sulphate from a natural vegan source, formulate it into tablets, and evaluate its physicochemical and pharmaceutical properties. Glucosamine hydrochloride was extracted from the cell wall of Agaricus bisporus (A. bisporus) mushrooms by converting chitin into chitosan, followed by enzymatic hydrolysis with α-amylase and glucoamylase. The hydrolyzed product was subsequently converted into glucosamine sulphate and characterized using FTIR, PXRD, thermal analysis, microscopic examination, and micromeritic studies. The compound was further formulated into tablets and evaluated for pharmaceutical performance. Glucosamine sulphate was successfully synthesized from A. bisporus, with an overall yield of 2% from wet biomass. FTIR confirmed the structural identity, while PXRD established its crystalline nature. The product exhibited low hygroscopicity (0.2% mo555isture content) and good thermal stability, as indicated by DSC. Micromeritic studies showed acceptable flow and compressibility properties, supporting suitability for tableting. Coated tablets demonstrated efficient drug release, achieving \~90% dissolution within 60 minutes. Glucosamine sulphate synthesized from A. bisporus mushrooms showed satisfactory yield, desirable physicochemical properties, and rapid dissolution, highlighting its potential as a sustainable natural source for the prevention and management of osteoarthritis.