Highly sensitive luciferase-based assay with red fluorescent protein expression for accurate quantitative monitoring and real-time visualization of cell invasion
Overall, using luciferase and dTomato co-expressing cells for invasion assay showed reliable and accurate measurements of variations in cell invasion patterns. Introducing these cells reduced time-consuming steps, improved sensitivity, and endpoints measurements, while being capable of real-time visualization, providing advantages over traditional methods.
Authors
- Jesper T. Troelsen (ORCID: https://orcid.org/0000-0002-4177-3267)
- Stine Bull Jessen (ORCID: https://orcid.org/0000-0003-0925-9484)
- Frederik Diness (ORCID: https://orcid.org/0000-0001-5098-7198)
- Ismail Gögenür (ORCID: https://orcid.org/0000-0002-3753-268X)
- Tove Kirkegaard
- Michael Christensen
Institutions
- Roskilde University (DK)
- Zealand University Hospital (DK)
- Zealand University Hospital Køge (DK)
Publication Details
- Journal
- Analytical Biochemistry
- Published
- 2025-09-27
- DOI
- https://doi.org/10.1016/j.ab.2025.115986
- Primary Topic
- Cellular Mechanics and Interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.00