Wasabi leaf extract changes the expression levels of cytokine-related genes in dermal papilla cells, as identified through whole transcriptome analysis

Wasabi is a traditional Asian food, and it has been reported that Wasabi leaf extract derived compounds, such as 6-methylsulfinylhexyl isothiocyanate (6-MSITC), exert anti-proliferative effects on cancer cells. In addition, although the antibacterial action of wasabi leaf extract has been reported, the detailed biological effects of wasabi leaf extract are not fully understood. To obtain further associated information, we used next-generation sequencing to identify upregulated or downregulated genes in immortalized human dermal papilla cells, and differentially expressed genes in human papilla cells were identified in the presence or absence of wasabi leaf extract. The effects of 6-MSITC and isosaponarin were also evaluated. The results indicated that wasabi leaf extracts upregulated or downregulated the expression of cytokine-related genes, such as GDF15, BMP6, CXCL12. The expression changes of GDF15, BMP6 were reproduced by real time PCR, and the results will contribute to our understanding of the biological effects of wasabi.