Three novel Luteimonas species from faeces of Cygnus atratus: Luteimonas changyuni sp. nov., Luteimonas yanweni sp. nov. and Luteimonas qiangzhengi sp. nov.
Six Gram-stain-negative, aerobic and rod-shaped bacterial strains, designated as MJ145<sup>T</sup>, MJ204, MJ174<sup>T</sup>, MJ246, MJ146<sup>T</sup> and MJ293, were isolated from faeces of <i>Cygnus atratus</i>, in Qinghai, PR China. The six strains grew at pH 7.0-9.0, 4-37 °C; strains MJ145<sup>T</sup>, MJ204, MJ174<sup>T</sup> and MJ246 grew at 0-6.0% NaCl; MJ146<sup>T</sup> and MJ293 grew at 0-5.0% NaCl. The 16S rRNA gene sequence analysis revealed that strains MJ145<sup>T</sup> and MJ204 exhibited the highest similarity (98.21% and 98.03 %, respectively) to <i>Luteimonas terricola</i> BZ92r<sup>T</sup>, strains MJ174<sup>T</sup> and MJ246 showed the highest similarity (98.36% and 98.16 %, respectively) to '<i>Luteimonas granuli</i>' Gr-4 and strains MJ146<sup>T</sup> and MJ293 showed the highest similarity (96.36% and 96.38 %, respectively) to '<i>Luteimonas dalianensis</i>' OB44-3. Combined with the phylogenomic tree, it showed that strain pairs MJ145<sup>T</sup>/MJ204 and MJ174<sup>T</sup>/MJ246 were most closely related to <i>L. terricola</i> BZ92r<sup>T</sup>, while strain pair MJ146<sup>T</sup>/MJ293 was most closely related to '<i>L. dalianensis</i>' OB44-3, <i>Luteimonas suaedae</i> LNNU 24178<sup>T</sup>, <i>Luteimonas salinilitoris</i> C3-2-a3<sup>T</sup>, <i>Luteimonas endophytica</i> RD2P54<sup>T</sup> and <i>Luteimonas salinisoli</i> SJ-92<sup>T</sup>. The average nt identity and digital DNA-DNA hybridization values between the strains and the other members of the genus <i>Luteimonas</i> were below the established thresholds of 95-96% and 70 %, respectively. The respiratory ubiquinone-8 was detected in the strains MJ145<sup>T</sup>, MJ174<sup>T</sup> and MJ146<sup>T</sup>. The major fatty acids of all three strains (MJ145<sup>T</sup>, MJ174<sup>T</sup> and MJ146<sup>T</sup>) were iso-C<sub>15 : 0</sub> and summed feature 9 (C<sub>16 : 0</sub> 10-methyl and/or iso-C<sub>17 : 1</sub> <i> ω</i>9c). All three strains contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. Based on polyphasic characterization, these six strains should be considered to be three novel species within genus <i>Luteimonas</i>, for which the names <i>Luteimonas changyuni</i> sp. nov. (MJ145<sup>T</sup>/MJ204, type strain MJ145<sup>T</sup>=GDMCC 1.4700<sup>T</sup>=JCM 37394<sup>T</sup>), <i>Luteimonas yanweni</i> sp. nov. (MJ174<sup>T</sup>/MJ246, type strain MJ174<sup>T</sup>=GDMCC 1.4862<sup>T</sup>=JCM 37396<sup>T</sup>) and <i>Luteimonas qiangzhengi</i> sp. nov. (MJ146<sup>T</sup>/MJ293, type strain MJ146<sup>T</sup>=GDMCC 1.4701<sup>T</sup>=JCM 37395<sup>T</sup>) are proposed, respectively.
Authors
- Lili Ren (ORCID: https://orcid.org/0000-0002-6645-8183)
- Jianguo Xu (ORCID: https://orcid.org/0000-0003-3649-8229)
- Ji Pu (ORCID: https://orcid.org/0000-0003-2268-9796)
- Wenbo Luo (ORCID: https://orcid.org/0000-0001-8067-6681)
- Jinlv Liu (ORCID: https://orcid.org/0009-0000-1189-9953)
- Qirong Xiao (ORCID: https://orcid.org/0000-0001-9419-3939)
- Hao Zhou (ORCID: https://orcid.org/0000-0002-9256-6022)
- H. J. Yang (ORCID: https://orcid.org/0009-0004-8274-9863)
- Han Wang (ORCID: https://orcid.org/0000-0003-2420-3147)
- Yuqing Liu (ORCID: https://orcid.org/0000-0002-6324-1296)
- Qiang Hou (ORCID: https://orcid.org/0000-0002-7874-454X)
- Jiajia Ma
Institutions
- Shanxi Medical University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Peking Union Medical College Hospital (CN)
- National Institute for Communicable Disease Control and Prevention (CN)
- Nanjing Medical University (CN)
Publication Details
- Journal
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Published
- 2025-09-22
- DOI
- https://doi.org/10.1099/ijsem.0.006923
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00