Plastid RNA Editing in Glycyrrhiza uralensis: Landscape Characterization and Comparative Assessment of RNA-Seq Library Strategies for Detection

We establish a set of 38 high-confidence plastid RNA editing sites in <i>G. uralensis</i> and suggest potential adaptive implications of editing in <i>ndh</i>-related genes. Methodologically, total RNA-seq is recommended for identification using de novo RNA editing due to its high sensitivity and low false-positive rate; publicly available poly(A)-selected mRNA-seq datasets can be repurposed to reliably retrieve plastid RNA editing sites when stringent strand-specific filtering is applied.