Achieving Near‐Infrared Organic Room‐Temperature Phosphorescence for High‐Resolution Immune Response Monitoring and Bioimaging
Organic near-infrared (NIR) room-temperature phosphorescent (RTP) materials hold great potential for bioimaging due to their ability to eliminate background noise and tissue autofluorescence. Here, we synthesized octa-ring fused RTP molecules (TPP-BN and TPP-BF) with B─N coordination bonds via a two-step reaction, enabling NIR phosphorescent emission at 819 nm and a 28.6 ms lifetime. Using PMMA-b-PEG as host and surfactant to stabilize the RTP molecules, we fabricated PMMA-b-PEG based nanoparticles (PNPs) with five-fold brighter afterglow than conventional F127-based methods (FNPs). We further developed a granzyme B (GrB)-responsive nanoprobe (Q-BFNP) that achieves specific and quantitative detection. In vivo studies demonstrated their ability to monitor and distinguish tumor immune response with the signal-to-background ratio (SBR) as high as 216.4. This study provides a new method for constructing NIR organic RTP probes and advances applications of RTP materials in real-time, high-contrast bioimaging and tumor immune monitoring.
Authors
- Zitong Liu (ORCID: https://orcid.org/0000-0003-1185-9219)
- Yuping Dong (ORCID: https://orcid.org/0000-0001-7437-0678)
- Xianbin Ma
- Hai‐Yan Xie (ORCID: https://orcid.org/0000-0002-6330-7929)
- Bin Tong (ORCID: https://orcid.org/0000-0001-6896-5106)
- Xiaoyuan Huang (ORCID: https://orcid.org/0000-0002-7684-6294)
- Jiamin Qu
- Baicheng Mei (ORCID: https://orcid.org/0000-0003-0289-248X)
- Gengchen Li
- Jianbing Shi (ORCID: https://orcid.org/0000-0002-4847-1472)
- Yeyun Zhao
- Zhengxu Cai (ORCID: https://orcid.org/0000-0003-0239-9601)
- Shisheng Cui
- Kai Zhang (ORCID: https://orcid.org/0000-0001-5674-5579)
- Yongfeng Zhang (ORCID: https://orcid.org/0000-0003-2730-3549)
- Yuqi Wen
- Peng Sun (ORCID: https://orcid.org/0000-0001-8383-6513)
- Tao Wang (ORCID: https://orcid.org/0009-0003-1825-0163)
Institutions
- Beijing Institute of Technology (CN)
- China Pharmaceutical University (CN)
- Peking University (CN)
- Beijing University of Technology (CN)
- Beijing Research Institute of Mechanical and Electrical Technology (CN)
- Zero to Three (US)
- Lanzhou University (CN)
Publication Details
- Journal
- Angewandte Chemie International Edition
- Published
- 2025-09-26
- DOI
- https://doi.org/10.1002/anie.202511784
- Citations
- 6
- Primary Topic
- Luminescence and Fluorescent Materials
- Type
- article
- Field-Weighted Citation Impact
- 3.11