Effects of resveratrol on inflammatory and hormonal disorders induced by Helicobacter pylori OMVs in pregnant mice

Helicobacter pylori (H. pylori) is a type of pathogenic bacteria that can live in the host's stomach for a long time. H. pylori infection affects 50% of the world's population. Outer membrane vesicles (OMVs) secreted by H. pylori can enter the host blood circulation and participate in mediating systemic diseases. However, the effects of OMVs exposure during pregnancy on the inflammatory response and hormone metabolism remain unclear. H. pylori was cultured and its outer membrane vesicles were isolated. The structure and morphology of OMVs were observed by transmission electron microscopy and characterized by LC-MS. E18-19 pregnant mice were injected with OMVs via the tail vein to establish the exposure model and IL-6, IL-1β, TNF-α in the serum of pregnant mice after intragastric administration of Resveratrol (Res) were determined. Targeted metabolomics was used to detect the metabolic changes of related hormones in vivo after Res intervention. In vivo injection of Res can significantly reduce the serum level of IL-6, IL-1β and TNF-α attributable to H. pylori OMVs, improve the imbalance of androgens and steroids caused by H. pylori OMVs and regulate the level of estrogen. Res can inhibit the systemic inflammatory response caused by H. pylori OMVs and regulate the imbalance of androgen and steroid hormones caused by H. pylori OMVs.