Rapid and Sensitive Glucose Detection Using Recombinant Corn Mn Peroxidase and Advanced Voltammetric Methods

We present a novel disposable electrochemical biosensor for highly sensitive and selective glucose detection, employing gold-modified screen-printed electrodes combined with square wave (SWV) and linear sweep voltammetry (LSV). The sensor integrates recombinant corn-derived manganese peroxidase with glucose oxidase, bovine serum albumin, and gold nanoparticles to enhance stability and signal transduction. Glucose detection by LSV covered 0.001-6.5 mM (R² = 0.9913; LOD = 0.50 µM), while SWV achieved a broader range of 0.0006-6.5 mM (R² = 0.998; LOD = 0.29 µM). The sensor demonstrated excellent selectivity, showing minimal interference from common electroactive species including caffeine, aspartame, and ascorbic acid, and provided rapid responses, making it ideal for point-of-care and food monitoring applications.