Engineering Lanthanide Ion-Labeled Aptamers for ICP-MS Profiling of Serum-Derived Extracellular Vesicles from Colorectal Cancer
Membrane proteins on extracellular vesicles (EVs) derived from clinical serum samples have emerged as promising biomarkers for cancer screening, early diagnosis and prognosis. However, high-throughput profiling of multiple EV proteins with high sensitivity and high selectivity is challenge for clinical applications. In this work, we synthesized a polymer capable of chelating a set of lanthanide ions labeled at the terminal of DNA aptamers. These lanthanide ions-labeled aptamers have high affinity for specific EV proteins. By utilizing ICP-MS, multiplex profiling was performed for four specific proteins on the surface of EVs with high sensitivity and selectivity for CRC diagnosis. Precise diagnosis of EVs from CRC patients were achieved with an accuracy of 89.9%, sensitivity of 86.3%, and specificity of 100%. This method features high sensitivity, low background, and rapid detection, demonstrating potential clinical application for in vitro diagnosis with blood samples.
Authors
- Wang Xie (ORCID: https://orcid.org/0000-0001-5793-4881)
- Ziyan Du
- Ruizi Peng (ORCID: https://orcid.org/0000-0002-9388-7530)
- Weihong Tan (ORCID: https://orcid.org/0000-0002-8066-1524)
- Xiaobo Yu (ORCID: https://orcid.org/0000-0003-2352-9866)
- Yawei Feng (ORCID: https://orcid.org/0000-0002-9184-7260)
- Xiaoling Zheng (ORCID: https://orcid.org/0000-0002-5884-878X)
- Hubin Chen (ORCID: https://orcid.org/0009-0003-0216-7138)
- Yunshan Yang (ORCID: https://orcid.org/0000-0002-8148-3459)
- Cong Luo (ORCID: https://orcid.org/0000-0002-0879-3127)
- Y. Yang (ORCID: https://orcid.org/0000-0002-8917-2620)
- Jiaxuan He (ORCID: https://orcid.org/0009-0000-8797-4606)
- Zhiguang Zhou (ORCID: https://orcid.org/0000-0002-0374-1838)
Institutions
- Shanghai Jiao Tong University (CN)
- Chinese Academy of Sciences (CN)
- Renji Hospital (CN)
- Zhejiang Cancer Hospital (CN)
- Institute of Molecular Medicine (IN)
- Beijing Proteome Research Center (CN)
- Shaoxing People's Hospital (CN)
- Zhejiang University of Technology (CN)
Publication Details
- Journal
- JACS Au
- Published
- 2025-09-25
- DOI
- https://doi.org/10.1021/jacsau.5c00641
- Citations
- 1
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.81