Standardization of preclinical methodologies for discovery and validation of circulating microRNA biomarkers for post-traumatic epileptogenesis – Lessons learned from the EpiBioS4Rx Project 1
Our study demonstrates that conducting a successful plasma miRNA biomarker analysis requires procedural harmonization between laboratories, protocol standardization, inclusion and analysis of quality controls, training of researchers, and continuous monitoring of adherence to pre-agreed protocols.
Authors
- Pedro Andrade (ORCID: https://orcid.org/0000-0001-9118-8341)
- Gregory S. Smith (ORCID: https://orcid.org/0000-0002-2892-5481)
- Terence John O'Brien (ORCID: https://orcid.org/0000-0002-7198-8621)
- Mette Heiskanen (ORCID: https://orcid.org/0000-0003-1624-5240)
- Rhys D. Brady
- Idrish Ali (ORCID: https://orcid.org/0000-0002-0971-4654)
- Cesar Santana‐Gomez (ORCID: https://orcid.org/0000-0001-9163-5615)
- Nigel C. Jones (ORCID: https://orcid.org/0000-0002-1080-8439)
- Xavier Ekolle Ndode‐Ekane (ORCID: https://orcid.org/0000-0001-5682-5632)
- Neil G. Harris (ORCID: https://orcid.org/0000-0002-1965-6750)
- Meheli Banerjee
- Riikka Immonen (ORCID: https://orcid.org/0000-0002-9855-7098)
- Olli Gröhn (ORCID: https://orcid.org/0000-0003-1372-1651)
- Noora Puhakka (ORCID: https://orcid.org/0000-0001-6151-3510)
- Pablo M. Casillas‐Espinosa (ORCID: https://orcid.org/0000-0002-6199-9415)
- David Wright (ORCID: https://orcid.org/0000-0002-7535-8651)
- Sandy R. Shultz (ORCID: https://orcid.org/0000-0002-2525-8775)
- Glenn R. Yamakawa (ORCID: https://orcid.org/0000-0003-3079-6532)
- Richard J. Staba (ORCID: https://orcid.org/0000-0003-2285-5627)
- Emma L. Braine
- Asla Pitkänen (ORCID: https://orcid.org/0000-0002-8163-5416)
- Juliana Silva (ORCID: https://orcid.org/0000-0002-5546-932X)
- Matthew R. Hudson (ORCID: https://orcid.org/0000-0001-5016-735X)
- Shalini Das Gupta (ORCID: https://orcid.org/0000-0002-3613-0398)
Institutions
- University of California, Los Angeles (US)
- Monash University (AU)
Publication Details
- Journal
- Epilepsy Research
- Published
- 2025-09-22
- DOI
- https://doi.org/10.1016/j.eplepsyres.2025.107667
- Citations
- 1
- Primary Topic
- MicroRNA in disease regulation
- Type
- article
- Field-Weighted Citation Impact
- 0.94