Low-Cost Sucrose Gradient Protocol for GFP Purification from Recombinant E. coli: A Visual Fluorescence-Based Method for Resource-Limited Laboratories v1

This protocol describes a simple, cost-effective method for purifying Green Fluorescent Protein (GFP) from E. coli using a sucrose gradient. It avoids toxic chemicals and expensive chromatography systems, making it accessible and environmentally friendly. The method preserves GFP's fluorescence and is suitable for applications in biomedical imaging, molecular tagging, and gene expression education laboratories. with further improvements, it could be used in research studies.

Authors

Publication Details

Published
2025-09-26
DOI
https://doi.org/10.17504/protocols.io.n92ld62pxg5b/v1
Primary Topic
Cell Image Analysis Techniques
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Low-Cost Sucrose Gradient Protocol for GFP Purification from Recombinant E. coli: A Visual Fluorescence-Based Method for Resource-Limited Laboratories v1

Joseph shenekji, Kamar Shayah, Suad Ali Hassna, Wjoud Kahel et al.
Cell Image Analysis Techniques
article

Low-Cost Sucrose Gradient Protocol for GFP Purification from Recombinant E. coli: A Visual Fluorescence-Based Method for Resource-Limited Laboratories v1

Joseph shenekji, Kamar Shayah, Suad Ali Hassna, Wjoud Kahel, Hind Alnaem, Najwa Hasn Bek
article en

Abstract

This protocol describes a simple, cost-effective method for purifying Green Fluorescent Protein (GFP) from E. coli using a sucrose gradient. It avoids toxic chemicals and expensive chromatography systems, making it accessible and environmentally friendly. The method preserves GFP's fluorescence and is suitable for applications in biomedical imaging, molecular tagging, and gene expression education laboratories. with further improvements, it could be used in research studies.

Openalex Percentile: Top 81%
Cell Image Analysis Techniques
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Low-Cost Sucrose Gradient Protocol for GFP Purification from Recombinant E. coli: A Visual Fluorescence-Based Method for Resource-Limited Laboratories v1 — Joseph shenekji, Kamar Shayah, et al. · (2025) | TGRS Research Map | TGRS