Large-scale screening of genes responsible for silique length and seed size in Brassica napus via pooled CRISPR library
This study harnessed the CRISPR/Cas9 technology to establish a preliminary library of gene-edited mutants in B. napus, thereby laying a robust foundation for the future screening of candidate genes pertaining to silique development and seed size. Furthermore, this study provides a methodological framework for rapid functional gene discovery in B. napus through CRISPR-based approaches.
Authors
- Yixian Song
- Chenqi Zhao
- Jie Xia (ORCID: https://orcid.org/0000-0003-4574-9376)
- Baoling Liang (ORCID: https://orcid.org/0009-0002-2198-8743)
- Dengfeng Hong (ORCID: https://orcid.org/0000-0001-9460-219X)
- Jianwei Gu
- Shengzhe Lin (ORCID: https://orcid.org/0009-0006-6241-6708)
Institutions
- Huazhong Agricultural University (CN)
- Hubei Engineering University (CN)
Publication Details
- Journal
- BMC Genomics
- Published
- 2025-09-26
- DOI
- https://doi.org/10.1186/s12864-025-11979-y
- Citations
- 1
- Primary Topic
- Photosynthetic Processes and Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Huazhong Agricultural University
- National Science and Technology Major Project